Inhibition of neuraminidase activity by derivatives of 2-deoxy-2,3-dehydro- N -acetylneuraminic acid
Identifieur interne : 002C10 ( Main/Exploration ); précédent : 002C09; suivant : 002C11Inhibition of neuraminidase activity by derivatives of 2-deoxy-2,3-dehydro- N -acetylneuraminic acid
Auteurs : P. Meindl [Autriche] ; G. Bodo [Autriche] ; P. Palese [États-Unis] ; J. Schulman [États-Unis] ; H. Tuppy [Autriche]Source :
- Virology [ 0042-6822 ] ; 1974.
English descriptors
- Teeft :
- Academic press, Acid, Amino group, Bacterial sources, Carbon atom, Cell receptors, Cholerae, Cholerae neuraminidase, Derivative, Different compounds, Different substitutions, Enzyme inhibition, Fetuin, Hemagglutination, Hemagglutination inhibition, Hon2, Influenza, Influenza virus, Influenza virus neuraminidase, Inhibition, Inhibitor, Inhibitor concentration, Inhibitory activity, Meindl, Methyl esters, Myxovirus, Nacetylneuraminic acid, Neuraminic, Neuraminic acid analogs, Neuraminic acid derivatives, Neuraminidase, Neuraminidase activity, Neuraminidase inhibition, Neuraminidase inhibitors, Neuraminidases, Newcastle disease virus, Palese, Parainfluenza virus type, Parainfluenza viruses, Phosphate buffer, Potent neuraminidase inhibitor, Reaction mixture, Sendai virus, Standard procedures, Synthetic inhibitors, Tissue culture, Tuppy, Unpublished results, Vibrio cholerae neuraminidase, Viral, Viral neuraminidases, Weight substrate.
Abstract
Abstract: Eighteen derivatives of 2-deoxy-2,3-dehydro-N-acetylneuraminic acid were assayed for inhibitory activity against neuraminidases from viral and bacterial sources. Twelve of these compounds were active against neuraminidases of Vibrio cholerae, influenza AMel, BLee, and Newcastle disease virus, causing 50% enzyme inhibition in concentrations ranging from 10−3M to 10−6M. The most active of them and the most potent neuraminidase inhibitor described so far is 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid. This compound has an inhibitor constant (Ki) of 7,9 × 10−7, M for influenza AMel virus neuraminidase whereas the Km of the virus enzyme for the substrate is 1000 times weaker (Km = 6, 9 × 10−4M). The mechanism of inhibition is competitive, and enzyme inhibition is independent of enzyme concentration. 2-Deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid inhibits hemagglutination by NDV and SV5 but does not inhibit agglutination of red cells by Sendai virus or influenza A and B viruses.
Url:
DOI: 10.1016/0042-6822(74)90080-4
Affiliations:
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Twelve of these compounds were active against neuraminidases of Vibrio cholerae, influenza AMel, BLee, and Newcastle disease virus, causing 50% enzyme inhibition in concentrations ranging from 10−3M to 10−6M. The most active of them and the most potent neuraminidase inhibitor described so far is 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid. This compound has an inhibitor constant (Ki) of 7,9 × 10−7, M for influenza AMel virus neuraminidase whereas the Km of the virus enzyme for the substrate is 1000 times weaker (Km = 6, 9 × 10−4M). The mechanism of inhibition is competitive, and enzyme inhibition is independent of enzyme concentration. 2-Deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid inhibits hemagglutination by NDV and SV5 but does not inhibit agglutination of red cells by Sendai virus or influenza A and B viruses.</div></front></TEI><affiliations><list><country><li>Autriche</li><li>États-Unis</li></country><region><li>Vienne (Autriche)</li><li>État de New York</li></region><settlement><li>Vienne (Autriche)</li></settlement></list><tree><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Meindl, P" sort="Meindl, P" uniqKey="Meindl P" first="P." last="Meindl">P. Meindl</name></region><name sortKey="Bodo, G" sort="Bodo, G" uniqKey="Bodo G" first="G." last="Bodo">G. Bodo</name><name sortKey="Tuppy, H" sort="Tuppy, H" uniqKey="Tuppy H" first="H." last="Tuppy">H. 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